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KMID : 0357419960260020163
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Korean journal of Virology
1996 Volume.26 No. 2 p.163 ~ p.172
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Immunogenicity of Recombinant Pseudorabies Virus Major Capsid Protein Expressed by Baculovirus Vector System
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전무형
안동준/장경수/조용성/박종현/송재영/현방훈/안수환
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Abstract
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The recombinant pseudorabies virus major capsid protein (rMCP) was produced by expression of the MCP gene in Sf-9 cell using baculovirus transfer vector system. Following evaluation of the immunochemical properties of the rMCP, the immunogenicity
of the
recombinant subunit protiens were investigated in guinea pig and swine to obtain the preliminary guide line for the subunit vaccine using rMCP and gP50.
It was proved that ultrasonication and 30% ammonium sulfate was most efficient to concentrate and purify the protein. The rMCP was safe in mice, guinea pigs and piglets. In guineapigs, rMCP mixed with various adjuvants induced substantial degree
of
serum neutralizing antibody titers, but revealed incomplete protectivity against challenge. In swine, the combination of rMCP and gP50 showed the higher serum neutralizing antibody titers and cellular immune responses than rMCP alone. However,
the
protectivity was lower in comparison with the commercial gI-deleted inactivated vaccine.
We expect these results to contribute to characterization of MCP gene of Korean isolate of PRV and to ultilize as preliminary information for prodution and evaluation of PRV recombinant subunit vaccines.
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KEYWORD
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